20:30-24:00

Chair: Eric O. Freed (NIH/NCI)

19:30-19:50 Kelly K. Lee (Univ of Washington) Cryo-electron tomography of Env on intact HIV virions reveals the variable nature of the ectodomain tether and membrane proximal external region

19:50-20:10 Maolin Lu (Univ of Texas HSC at Tyler) An intact amber-free HIV-1 system for in-virus protein bioorthogonal click labeling that delineates envelope conformational dynamics

20:10-20:30 Paul Spearman (Cincinnati Children's Hospital) Tubular Recycling Endosome Directs Recycling of the HIV-1 Envelope Glycoprotein Complex and is Required for Cytoplasmic Tail-Dependent Incorporation into Particles

Social Hour

Co-Chairs: Karin Musier-Forsyth (Ohio State University) and Kathleen Boris-Lawrie (University of Minnesota)

9:20-9:40 Xiao Heng (Univ of Missouri) HIV cap epigenetic modification is regulated by host DHX9/RHA

9:40-10:00 Sebla B. Kutluay (Washington Univ, St Louis) Efficient packaging of HIV-1 gRNA by heterologous RNA-binding domains

10:00-10:20 Ioulia Rouzina (Ohio State Univ) Kinetic model of high packaging selectivity of the dimeric HIV-1 genomic RNA

11:00-11:20 Wei-Shau Hu (NIH) HIV-1 Usurps Transcription Start Site Heterogeneity of Host RNA Polymerase II to Maximize Replication Fitness

11:20 -11:40. Takeshi Yoshida (National Institute of Infectious Diseases, Japan) HIV-1 RNAs preferentially used as genomic RNA for generating provirus DNA

11:40-11:50 (Short) Zetao (Chris) Cheng (NIH) Role of Transcriptional Start Site Heterogeneity in HIV-1 Unspliced RNA Translation: Cell-based Studies

11:50-12:00 (Short) Joseph G. Kanlong (Ohio State Univ) Role of transcriptional start site heterogeneity in HIV-1 unspliced RNA translation: in vitro studies

Chair: Delphine Muriaux (IRIM/CNRS, France)

15:20 -15:40 Akira Ono (Univ of Michigan) HIV-1 Gag interaction with tRNA via MA-HBR

15:40-16:00 Serena Bernacchi (IBMC, CNRS, France) Characterization of the molecular factors regulating the interactions between HIV-1 Gag precursor and genomic RNA in viral assembly reconstitution

16:00 -16:15 (15 min) Tomáš Ruml (Univ of Chemistry and Technology, Prague) Myristoyl switch at the plasma membrane serves as the trigger for Gag cleavage and oligomerization of the matrix protein in the Mason-Pfizer monkey virus.

16:15-16:25 (Short) Puja Banerjee (Univ of Chicago) Understanding the HIV-1 Gag Protein Assembly Process at the Plasma Membrane

16:25-16:35 (Short) Nario Tomishige (Univ of Strasbourg) HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.

Chair: Barbara Müller (Center for Integrative Infectious Disease Research, Germany)

17:15 -17:35 Louis Mansky (Univ of Minnesota) New insights into human retrovirus particle assembly and release

17:35-17:55 Alan Rein (NIH) Dissection of HIV-1 immature particle assembly

17:55-18:15 Francesca Di Nunzio (Institut Pasteur, Université Paris Cité) Biomolecular condensates and HIV infection

18:15 -18:25 (Short) Abby Peterson (Univ of Utah)  Deep sequencing of HIV virions released from cells with competing HIV backbones suggests that HIV assembly is initiated by Gag molecules bound to their parental gRNA after translation

Chair: Julia Kenyon (University of Cambridge)

9:20-9:40 Till Böcking (UNSW, Australia) Pharmacologic hyperstabilisation of the HIV-1 capsid lattice induces capsid failure

9:40-10:00 Kathleen Boris-Lawrie (Univ of Minnesota) HIV cap epigenetic modification is inhibited by ABX46

Chair: Mark Williams (Northeastern University)

10:40-11:00. Barbara Müller (University Hospital Heidelberg, Germany) Imaging analysis of the post entry phase of retroviral replication

11:00-11:15 (15 min) Felipe Diaz-Griffero (Albert Einstein College of Medicine) Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating in Cycling and Non-cycling Cells

11:15-11:30 (15 min) João I Mamede (Rush Univ) Fluorescent labeled CA correlates progressive uncoating from the cytoplasm to the nucleus to productive HIV infection in primary cells.

11:30-11:45 (15 min) Juan R. Perilla (Univ of Delaware) HIV-1 capsid mechanoelastic properties regulate nuclear import and uncoating

11:45-11:55 (Short) Michael Morse (Northeastern Univ) AFM measurements of nucleic acid conformation demonstrate that nucleocapsid protein-mediated condensation is critical to capsid stability

11:55-12:05 (Short) Ryan Burdick (NIH) Reverse transcription of long double-stranded DNA triggers HIV-1 uncoating

Chair: Till Böcking (UNSW, Australia)

16:50-17:10 Owen Pornillos (Univ of Utah) An HIV-1 capsid assembly switch and its implications for capsid function

17:10-17:20 (Short) Lydia M. Arnold (Univ of Utah) In vitro reconstitution of HIV-1 reverse transcription, capsid destabilization, and innate immune sensing via cGAS

17:20-17:30 (Short) Alex B. Kleinpeter (NIH) Manipulation of the central pore in capsomers provides key insights into the role of IP6 in HIV-1 capsid function

Ileana M. Cristea (Princeton University) The virus microenvironment: Intra- and inter-cellular communication during infection

Chair: Saveez Saffarian (University of Utah)

9:20-9:40 Dominik Hrebík (Max Planck Institute of Biochemistry, Germany) High resolution in situ structures of HIV-1 matrix protein

9:40 -9:55 (15 min) Rachna Aneja (Univ of Missouri) RNA Aptamer that binds HIV-1 capsid lattice inhibits viral infection by interfering with capsid maturation

9:55-10:10 (15 min) Benjamin Preece (Univ. of Utah) Cryotomography analysis of NL4.3 (ᐃPol) virions

Chair: Wesley Sundquist (University of Utah)

10:50-11:10 Nathan Sherer (Univ of Wisconsin) Host factors co-trafficking with HIV-1 RNA genomes

11:10-11:25 (15 min) Rumlová Michaela (Univ of Chemistry and Technology, Prague) Unravelling Betaretroviral Replication: The Pivotal Role of Host RNA Helicase DHX15

11:25-11:35 (Short) Stephanie Ullrich (Univ Hospital Heidelberg, Germany) A novel pulse-chase fluorescence imaging approach for the analysis of HIV-1 cell-to-cell transmission and spread

11:35-11:45 (Short) Micah McCauley (Northeastern Univ) Nucleosome Destabilization and Histone Loss Induced by HIV-1 Vpr

11:45 -12:00 Closing Remarks/Awards

Lunch: Enjoy the Oktoberfest!